Procedimiento sencillo para obtener grandes cantidades de antigenos del VIH para serodiagnostico
Fecha
s.d.1988
Metadatos
Mostrar el registro completo del ítemResumen
The established method of purifying human immunodeficiency virus (HIV) involves centrifuging the lysates of infected cells onto a sucrose gradient. We have developed an alternative methodology for purifying large amounts of HIV antigens that avoids the need for expensive equipment such as an ultracentrifuge and requires no purification gradient. These semipurified antigens have been use to perform a "homemade" confirmatory Western blot assay that produced results comparable to those obtained with a commercial (Du Pont) Western blot assay. Although the "homemade" procedure did not detect one important viral protein (env gp 160), this purification method seems to offer a suitable and economical way of obtaining antigens to confirm the presence of HIV antibodies
Título traducido
A simple procedure for obtaining large amount of HIV antigens for serodiagnostic purposes
Colecciones
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Brown, K. N (s.d.)In any experimental approach many factors will have to be considered and each parasite will set its own technical limitations. Nevertheless the points mentioned in this article are likely to contribute to a full analysis ...
-
Madalengoitia, José; Ishida, N; Umenay, T; Miyamoto, T; Mejía, J; Flores, William; Sánchez, S; Méndez, R (1975)Single radial immune diffusion and counterelectrophoresis tests were used to examine 2,593 serum speciments from apparently healthy men, women, and children of Peru for the presence of hepatitis B antigen (Australia antigen). ...
-
Dowdle, Walter Reid; Schild, G.C (1976)Influenza viruses have two surface antigens, the glycoprotein structures hemagglutinin (HA) and neuraminidase (NA). Antibodies to each of these are associated with immunity, but the structures themselves are antigenically ...