Susceptibilidad de Aedes aegypti a DDT, deltametrina y lambdacialotrina en Colombia
Date
2010Metadata
Show full item recordAbstract
OBJETIVOS: Evaluar el estado de susceptibilidad a insecticidas piretroides deltametrina y lambdacialotrina y al organoclorado DDT, e identificar los mecanismos bioquímicos asociados con resistencia en 13 poblaciones naturales de Aedes aegypti recolectadas en localidades de Colombia donde el dengue es un grave problema de salud pública. MÉTODOS: Se recolectaron y criaron en condiciones controladas formas inmaduras de diferentes criaderos naturales del vector para cada localidad. Con la generación F2 se realizaron bioensayos utilizando las metodologías OMS 1981 (papeles impregnados) y CDC 1998 (botellas impregnadas). En las poblaciones con mortalidades compatibles con disminución de la susceptibilidad, se midieron los niveles de esterasas no específicas (ENE), oxidasas de función mixta (OFM) y acetilcolinesterasa modificada (ACEM) mediante pruebas colorimétricas. RESULTADOS: Todas las poblaciones del mosquito evaluadas evidenciaron resistencia al organoclorado DDT. En cuanto a los piretroides, se encontró resistencia generalizada a lambdacialotrina pero no a deltametrina. Los mecanismos bioquímicos de resistencia evaluados permitieron encontrar 7 de 11 poblaciones con ENE elevadas y una población con OFM incrementadas. CONCLUSIONES: Se descarta la resistencia cruzada de tipo fisiológico entre el DDT y lambdacialotrina en las poblaciones de A. aegypti evaluadas. La resistencia fisiológica a lambdacialotrina parece asociarse con el incremento de las ENE. El comportamiento diferencial en los niveles de susceptibilidad y los valores enzimáticos entre poblaciones se asociaron con la variabilidad genética y presión de selección química a nivel local.(AU) OBJECTIVES: To assess the susceptibility status of 13 natural populations of Aedes aegypti (collected from sites in Colombia where dengue is a serious public health problem) to the pyrethroids, deltamethrin and lambda-cyhalothrin, and to the organochlorine, DDT, and to identify any biochemical mechanisms associated with resistance. METHODS: Immature forms of the vector were collected from natural breeding spots at each site and then raised under controlled conditions. Using the F2 generation, bioassays were performed using the World Health Organization's 1981 methodology (impregnated paper) and United States Centers for Disease Control and Prevention's 1998 methodology (impregnated bottles). In populations where mortality rates were consistent with decreased susceptibility, levels of nonspecific esterases (NSE), mixed-function oxidases (MFO), and acetylcholinesterase (AChE) were measured using colorimetric tests. RESULTS: All of the mosquito populations that were tested showed resistance to the organochlorine DDT. In the case of the pyrethroids, widespread resistance to lambda-cyhalothrin was found, but not to deltamethrin. Assessing the biochemical resistance mechanisms showed that 7 of the 11 populations had elevated NSE, and one population, increased MFO. CONCLUSIONS: Physiological cross-resistance between DDT and lambda-cyhalothrin in the A. aegypti populations tested was dismissed. Physiological resistance to lambda-cyhalothrin appears to be associated with increased NSE. The differences in susceptibility levels and enzyme values among the populations were associated with genetic variations and chemicals in use locally.(AU)
Subject
Aedes; DDT; Dengue; Insecticidas; Mosquito; Insecticidas Organoclorados; Resistencia a los insecticidas; Colombia; Aedes; DDT; Dengue; Insecticides; Mosquito control; Insecticides; Organochlorine; Insecticide Resistance; Colombia; Aedes; DDT; Inseticidas; Controle de Mosquitos; Nitrilos; Piretrinas; Acetilcolinesterase; Aedes; Bioensaio; Carboxilesterase; Colômbia; Colorimetria; Resistência a Medicamentos; Resistência a Múltiplos Medicamentos; Proteínas de Insetos; Oxigenases de Função Mista
URI
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892010000100010https://iris.paho.org/handle/10665.2/9577
Citation
Santacoloma Varón, Liliana,Chaves Córdoba, Bernardo,Brochero, Helena Luisa (2010) Susceptibilidad de Aedes aegypti a DDT, deltametrina y lambdacialotrina en Colombia. Rev Panam Salud Publica;27(1) 66-73,enero 2010. Retrieved from http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892010000100010
Collections
Related items
Showing items related by title, author, creator and subject.
-
Guagliardo, Sarah Anne J.; Ardila Roldan, Susana Carolina; Santacoloma, Liliana; Luna, Cesar; Cordovez Alvarez, Juan Manuel; Rojas Gacha, Juan David; Mansur, Mariana; Levine, Rebecca S.; Lenhart, Audrey; Fuya Oviedo, Patricia (2019)[ABSTRACT]. In the wake of the Zika epidemic, there has been intensified interest in the surveillance and control of the arbovirus vectors Aedes aegypti and Aedes albopictus, yet many existing surveillance systems could ...
-
Fox, Irving (s.d.)A laboratory colony of Aëdes aegypti established from specimens collected at Isla Verde near San Juan, Puerto Rico, was found to be highly resistant to certain chlorinated hydrocarbons and moderately resistant to some ...
-
Polson, Karen A.; Brogdon, William G.; Rawlins, Samuel C.; Chadee, Dave D. (2012)