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Impact of environmental temperatures on resistance to organophosphate insecticides in Aedes aegypti from Trinidad

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Date
2012
Author
Polson, Karen A.
Brogdon, William G.
Rawlins, Samuel C.
Chadee, Dave D.
Metadata
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Abstract
OBJECTIVE: To examine the effects of increasing larval rearing temperatures on the resistance status of Trinidadian populations of Aedes aegypti to organophosphate (OP) insecticides. METHODS: In 2007-2008, bioassays and biochemical assays were conducted on A. aegypti larvae collected in 2006 from eight geographically distinct areas in Trinidad (Trinidad and Tobago). Larval populations were reared at four temperatures (28 ± 2ºC, 32ºC, 34ºC, and 36ºC) prior to bioassays with OP insecticides (fenthion, malathion, and temephos) and biochemical assays for esterase enzymes. RESULTS: Most larval populations reared at 28 ± 2ºC were susceptible to fenthion (>98% mortality) but resistant to malathion and temephos ( 80% mortality). A positive association was found between resistance to OP insecticides and increased activities of α- and β-esterases in larval populations reared at 28 ± 2ºC. Although larval populations reared at higher temperatures showed variations in resistance to OPs, there was a general increase in susceptibility. However, increases or decreases in activity levels of enzymes did not always correspond with an increase or decrease in the proportion of resistant individuals reared at higher temperatures. CONCLUSIONS: Although global warming may cause an increase in dengue transmission, based on the current results, the use of insecticides for dengue prevention and control may yet be effective if temperatures increase as projected.(AU)
 
OBJETIVO: Examinar los efectos del aumento de las temperaturas de desarrollo larvario sobre el estado de resistencia a los insecticidas organofosforados de las poblaciones de Aedes aegypti en Trinidad. MÉTODOS: En 2007 y 2008 se llevaron a cabo ensayos biológicos y bioquímicos en larvas de A. aegypti recogidas en el 2006 de ocho áreas geográficamente separadas en Trinidad (Trinidad y Tabago). Las poblaciones larvarias se desarrollaron en cuatro temperaturas (28 ± 2 ºC, 32 ºC, 34 ºC y 36 ºC) antes de los ensayos biológicos con insecticidas organofosforados (fentión, malatión y temefós) y los análisis bioquímicos para las enzimas de esterasa. RESULTADOS: La mayoría de las poblaciones larvarias que se desarrollaron a 28 ± 2 ºC fueron susceptibles al fentión (mortalidad > 98%) pero resistentes al malatión y al temefós (mortalidad 80%). Se encontró una asociación positiva entre la resistencia a los insecticidas organofosforados y la mayor actividad de αy β-esterasas en las poblaciones larvarias que se desarrollaron a 28 ± 2 ºC. Aunque las poblaciones larvarias que se desarrollaron a temperaturas mayores mostraron variaciones en la resistencia a los organofosforados, hubo un aumento general de la sensibilidad. Sin embargo, los aumentos o las disminuciones en los niveles de actividad de las enzimas no siempre se correspondieron con un aumento o disminución en la proporción de individuos resistentes desarrollados a las temperaturas más altas. CONCLUSIONES: Aunque el recalentamiento del planeta puede causar un aumento de la transmisión del dengue, según los resultados de este estudio el uso de insecticidas para la prevención y el control del dengue todavía puede ser eficaz si las temperaturas aumentan según lo proyectado.(AU)
 
Series
Rev Panam Salud Publica;32(1),jul. 2012
Subject
Culicidae; Aedes; Mosquito control; Insecticide Resistance; Insecticides, organophosphate; Temperature; Trinidad and Tobago; Culicidae; Aedes; Control de Mosquitos; Resistencia a los insecticidas; Insecticidas Organofosforados; Temperatura Ambiental; Trinidad y Tobago; Aedes; Fention; Insetos Vetores; Resistência a Inseticidas; Inseticidas; Malation; Temefós; Temperatura Ambiente; Aedes; Aedes; Dengue; Esterases; Esterases; Aquecimento Global; Temperatura Alta; Proteínas de Insetos; Proteínas de Insetos; Insetos Vetores; Insetos Vetores; Resistência a Inseticidas; Larva; Larva; Especificidade da Espécie; Trinidad e Tobago
URI
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892012000700001
https://iris.paho.org/handle/10665.2/9300
Citation
Polson, Karen A.,Brogdon, William G.,Rawlins, Samuel C.,Chadee, Dave D. (2012) Impact of environmental temperatures on resistance to organophosphate insecticides in Aedes aegypti from Trinidad. Rev Panam Salud Publica;32(1) 1-8,july 2012. Retrieved from http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892012000700001
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  • Pan American Journal of Public Health

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