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Molecular characterization of Streptococcus pneumoniae serotype 1 invasive isolates in Colombia

dc.contributor.authorDuarte, Carolinaes_ES
dc.contributor.authorSanabria, Olgaes_ES
dc.contributor.authorMoreno, Jaimees_ES
dc.date.accessioned2015
dc.date.available2015
dc.date.issued2013es_ES
dc.identifier.citationDuarte, Carolina,Sanabria, Olga,Moreno, Jaime (2013) Molecular characterization of Streptococcus pneumoniae serotype 1 invasive isolates in Colombia. Rev Panam Salud Publica;33(6) 422-426,jun. 2013. Retrieved from http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892013000600006es_ES
dc.identifier.urihttp://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892013000600006es_ES
dc.identifier.urihttps://iris.paho.org/handle/10665.2/9166
dc.format.extentgrafes_ES
dc.format.extenttabes_ES
dc.relation.ispartofseriesRev Panam Salud Publica;33(6),jun. 2013es_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.subjectMolecular epidemiologyes_ES
dc.subjectElectrophoresis, Gel, Pulsed-Fieldes_ES
dc.subjectMultilocus sequence typinges_ES
dc.subjectColombiaes_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.subjectEpidemiología Moleculares_ES
dc.subjectElectroforesis en Gel de Campo Pulsadoes_ES
dc.subjectTipificación de secuencias multilocuses_ES
dc.subjectColombiaes_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.subjectColômbiaes_ES
dc.subjectEletroforese em Gel de Campo Pulsadoes_ES
dc.subjectTipagem de Sequências Multilocuses_ES
dc.subjectSorotipagemes_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.titleMolecular characterization of Streptococcus pneumoniae serotype 1 invasive isolates in Colombiaes_ES
dc.typeJournal articlesen_US
dc.rights.holderPan American Health Organizationen_US
dc.description.notesOBJECTIVE: To determine the genetic relationship between Streptococcus pneumoniae serotype 1 Colombian isolates recovered from invasive disease between 1994 and 2011 and recognized serotype 1 international clones. METHODS: A total of 135 S. pneumoniae serotype 1 isolates with epidemiological and antimicrobial susceptibility data (Clinical and Laboratory Standards Institute, 2012) were studied. The genetic relationship with recognized international clones was established by pulsed-field gel electrophoresis (PFGE) with SmaI restriction enzyme. Multilocus sequence typing (MLST) was standardized to determine the sequence type (ST) in seven isolates representing different clonal groups. Control and reference strain R6, and clones Sweden¹ ST217, Sweden¹ ST304, Sweden¹ ST306, and USA¹ ST615, were used. RESULTS: PFGE revealed that 89.7% of the isolates were associated with Sweden¹ ST306, 3.7% were associated with Sweden¹ ST304, and 6.6% were not clonally related. Using MLST, ST306 was confirmed in six isolates and ST304 in one. CONCLUSIONS: In contrast to Brazil and the United States, where clones Sweden¹ ST304 and ST227 prevail, invasive disease caused by S. pneumoniae serotype 1 in Colombia is principally associated with the dispersion of isolates related to clone Sweden¹ ST306.(AU)en_US
dc.description.notesOBJETIVO: Determinar la relación genética entre las cepas de Streptococcus pneumoniae serotipo 1 aisladas en Colombia en casos de enfermedad invasora entre 1994 y 2011 y los clones internacionales reconocidos del serotipo 1. MÉTODOS: Se estudiaron un total de 135 cepas de S. pneumoniae serotipo 1 de las que se tenían datos epidemiológicos y de sensibilidad a los antimicrobianos (Clinical and Laboratory Standards Institute, 2012). Se estableció su relación genética con los clones internacionales reconocidos mediante electroforesis en gel de campo pulsátil (PFGE) utilizando la enzima de restricción SmaI. Se estandarizó la tipificación de secuencias mulitlocus (MLST) para determinar el tipo de secuencia (ST) en siete cepas que representaban diferentes grupos clonales. Se utilizaron la cepa de control y referencia R6 y los clones Sweden¹ ST217, Sweden¹ ST304, Sweden¹ ST306, y USA¹ ST615. RESULTADOS: La PFGE reveló que 89,7% de las cepas se asociaban con Sweden¹ ST306, 3,7% con Sweden¹ ST304, y 6,6% no mostraron relación clonal. Mediante MLST, se confirmó la relación con ST306 en seis cepas y con ST304 en una. CONCLUSIONES: A diferencia de Brasil y Estados Unidos, donde prevalecen los clones Sweden¹ ST304 y ST227, la enfermedad invasora causada por S. pneumoniae serotipo 1 en Colombia se asocia principalmente con la dispersión de cepas relacionadas con el clon Sweden¹ ST306.(AU)en_US


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