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dc.contributor.authorCampanha, Myriam T. Nes_ES
dc.contributor.authorHoshino-Shimizu, Sumiees_ES
dc.contributor.authorBaquerizo Martinez, Marinaes_ES
dc.date.accessioned2015
dc.date.available2015
dc.date.issued1999es_ES
dc.identifier.citationCampanha, Myriam T. N,Hoshino-Shimizu, Sumie,Baquerizo Martinez, Marina (1999) Urinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assay. Rev Panam Salud Publica;6(2) -,ago. 1999. Retrieved from http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49891999000700002&lng=pt&nrm=isoen_US
dc.identifier.urihttp://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49891999000700002&lng=pt&nrm=isoes_ES
dc.identifier.urihttps://iris.paho.org/handle/10665.2/8914
dc.format.extentiluses_ES
dc.relation.ispartofseriesRev Panam Salud Publica;6(2),ago. 1999es_ES
dc.subjectInfecções Urináriaspt_BR
dc.subjectEscherichia Colies_ES
dc.subjectBioensaioes_ES
dc.subjectTécnicas Imunoenzimáticases_ES
dc.subjectBrasilpt_BR
dc.titleUrinary tract infection: detection of Escherichia coli antigens in human urine with an ELIEDA immunoenzymatic assayen_US
dc.typeJournal articlesen_US
dc.rights.holderPan American Health Organizationen_US
dc.description.notesEscherichia coli is the most common causative agent of urinary tract infection (UTI), and diagnosing this infection usually relies on bacteriologic methods. Nevertheless, screening methods can be useful for a rapid presumptive diagnosis even though some of these screening methods have low sensitivity or are expensive. To investigate a possible new alternativa approach, an antigen-based immunoassay-enzyme-linked immunoelectrodiffusion assay (ELIEDA)- was standardized for screening for this bacterial infection. Combining counter-immunoelectrophoresis with an immunoenzymatic assay, the ELIEDA requires concentrated urine specimens, a cellulose acetate membrane, polyclonal antibodies to E. coli raised in rabbits, and peroxidase-labeled sheep antibodies to rabbit immunoglobulin G (IgG). This ELIEDA technique was evaluated using 244 urine specimens, 76 of them with E.coli, 47 with heterologous bacteria, and 121 without bacteria. In comparison to bacteriologic methods, the sensitivity, specificity, and positive and negative predictive values for the ELIEDA were 93.4 por ciento, 98.2 por ciento, 95.9 por ciento, and 97.1, respectively. The data obtained suggest that this assay is useful for routine diagnostic screening for UTI caused by E.coli. In addition, since the ELIEDA stained membranes can be stored, this assay makes retrospective studies possibleen_US


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