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In-house polymerase chain reaction for affordable and sustainable Chlamydia trachomatis detection in Trinidad and Tobago

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Date
2007
Author
Rampersad, Joanne
Wang, Xiaohui
Gayadeen, Helen
Ramsewak, Samuel
Ammons, David
Metadata
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Abstract
OBJECTIVES: To provide a preliminary assessment of in-house polymerase chain reaction (PCR) as an alternative to the more costly commercial test for detection of asymptomatic infection by Chlamydia trachomatis and to provide much needed demographic data on infection indicators within the Trinidad and Tobago public health care system. METHODS: An inexpensive in-house nested-PCR with an Internal Amplification Control was used to detect C. trachomatis and Neisseria gonorrhoeae in urine samples collected from 273 apparently healthy, pregnant women from March-September 2004 in Trinidad, West Indies. Demographic information on participants was collected and subjected to statistical analyses. RESULTS: C. trachomatis was detected in 57/273 (21 percent) samples, of which 5 (2 percent) were also positive for N. gonorrhoeae. Infection correlated well with certain demographic parameters, with the highest incidence of C. trachomatis infection found among pregnant women that were single or of African descent. CONCLUSIONS: Given the lack of commercial tests in Trinidad, in-house PCR is an inexpensive alternative that can be used to detect asymptomatic infections of C. trachomatis and to provide demographic information needed for interventions by the public health care system.(AU)
 
OBJETIVOS: Hacer una evaluación preliminar de un sistema autóctono para la detección de la infección asintomática por Chlamydia trachomatis mediante la reacción en cadena de la polimerasa (RCP), como alternativa a los costosos sistemas comerciales, y ofrecer datos demográficos muy necesarios relacionados con los indicadores de esta infección en el sistema de salud pública de Trinidad y Tobago. MÉTODOS: Se empleó un sistema autóctono y económico de RCP anidada con control interno de la amplificación para la detección de C. trachomatis y Neisseria gonorrhoeae en muestras de orina de 273 mujeres embarazadas asintomáticas, entre marzo y septiembre de 2004 en Trinidad y Tobago, Indias Occidentales. Se obtuvo la información demográfica de las participantes y se sometió a análisis estadístico. RESULTADOS: Se detectó C. trachomatis en 57/273 (21 por ciento) muestras, de las cuales 5 (2 por ciento) fueron también positivas para N. gonorrhoeae. La infección se correlacionó bien con algunos parámetros demográficos; la mayor incidencia de la infección por C. trachomatis se observó en las mujeres embarazadas solteras o de ascendencia africana. CONCLUSIONES: Debido al déficit de sistemas de diagnóstico comerciales en Trinidad, la RCP autóctona es una alternativa económica que puede emplearse para detectar la infección asintomática por C. trachomatis y obtener la información demográfica necesaria para que el sistema de salud pública implemente intervenciones.(AU)
 
Series
Rev Panam Salud Publica;22(5),nov. 2007
Subject
Chlamydia trachomatis; Polymerase Chain Reaction; Trinidad and Tobago; Chlamydia trachomatis; Reacción en cadena de la polimerasa; Trinidad y Tobago; Infecções por Chlamydia; Infecções por Chlamydia; Chlamydia trachomatis; Reação em Cadeia da Polimerase; Complicações Infecciosas na Gravidez; Complicações Infecciosas na Gravidez; Trinidad e Tobago
URI
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892007001000004
https://iris.paho.org/handle/10665.2/7764
Citation
Rampersad, Joanne,Wang, Xiaohui,Gayadeen, Helen,Ramsewak, Samuel,Ammons, David (2007) In-house polymerase chain reaction for affordable and sustainable Chlamydia trachomatis detection in Trinidad and Tobago. Rev Panam Salud Publica;22(5) 317-322,nov. 2007. Retrieved from http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892007001000004
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  • Pan American Journal of Public Health

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