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Desarrollo de una prueba ELISA para identificar anticuerpos antivirus de estomatitis vesicular Indiana-3

Development of an ELISA test to identify antibodies to vesicular stomatitis (Indiana-3) virus

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Español; 8 páginas (497.4Kb)
English; 8 pages (453.8Kb)
Date
1992
ISSN
0009-0131
Author
Allende, R.
Sepulveda, L. M.
Alonso, A.
Rangel Filho, F. B.
Organización Panamericana de la Salud
Metadata
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Abstract
La prueba ELISA de competición en fase líquida fue adaptada para la identificación de anticuerpos antivirus de estomatitis vesicular serotipo Indiana-3, usando como antígeno la glicoproteína viral. La validez y repetibilidad de la prueba se determinó por el estudio de 533 sueros de animales negativos y 305 sueros de animales positivos, obteniéndose una sensibilidad de 99 por ciento y una especificidad de 100 por ciento. No se observaron diferencias significativas entre las repeticiones de la prueba. Los valores predictivos de los resultados, positivo y negativo, y el valor global de la prueba estudiada indican que la ELISA es un método satisfactorio para identificar anticuerpos de estomatitis vesicular.
 
The competitive ELISA in liquid phase was adapted to identify antibodies to vesicular stomatitis Indiana-3 virus useing the viral glycoprotein as antigen. Test validity and reproducibility were determined by testing 553 sera from negative animals and 305 sera from positive animals, obtaining values of 99% sensitiviy and 100% specificity. No significant differences were observed among repeated tests. Prodictive values for positive and negative results, and the overall value of the technique studied indicate that it is a satisfactory method for use in the identification of antibodies to vesicular stomatitis virus.
 
Series
Bol Cent Panam Fiebre Aftosa;58
Subject
Virus de la Estomatitis Vesicular Indiana; Ensayo de Inmunoadsorción Enzimática; Anticuerpos Antivirales; Estomatitis Vesicular; Pruebas Serológicas; Vesicular stomatitis Indiana virus; Enzyme-Linked Immunosorbent Assay; Antibodies, Viral; Vesicular Stomatitis; Serologic Tests
URI
https://iris.paho.org/handle/10665.2/51286
Citation
Allende R, Sepulveda LM, Alonso A, Rangel Filho FB. Desarrollo de una prueba ELISA para identificar anticuerpos antivirus de estomatitis vesicular Indiana-3. Bol Cent Panam Fiebre Aftosa. 1992; 58: 55-62.
 
Allende R, Sepulveda LM, Alonso A, Rangel Filho FB. Development of an ELISA test to identify antibodies to vesicular stomatitis (Indiana-3) virus. Bol Cent Panam Fiebre Aftosa. 1992; 58: 63-70.
 
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  • Publications/Publicaciones - PANAFTOSA

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