Immunohistochemical recognition of a wide spectrum of lyssavirus in formalin-fixed tissues by one monoclonal antibody
Date
2001ISSN
0009-0131
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Automated indirect immunoperoxidase (avidin-biotin complex) staining using monoclonal antibody #5DF12-3B6, directed against rabies N-protein, was used to detect rabies antigen in tissue samples from animals either naturally or experimentally infected with rabies. This monoclonal antibody recognized all 16 strains of rabies virus tested, as well as rabies-related lyssaviruses including Duvenhage, Lagos Bat, and Mokola. The sample infected with Mokola virus initially showed only weak staining, however, deletion of protease digestion resulted in stronger stain uptake. The test was sensitive and specific, correctly identifying rabies antigen in all but one of the samples tested (37/38), and no apparent staining in any of the negative samples tested (23/23). Tissues from 16 mammalian species were tested, including one rabies infected human tissue sample. The utility of the immunoperoxidase staining method described in this study lies in the ability of one monoclonal to recognize a broad spectrum of lyssaviruses in formalin-fixed tissues.
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López JW, Trimarchi CV, Wandeler AI, Van Gessel Y. Immunohistochemical recognition of a wide spectrum of lyssavirus in formalin-fixed tissues by one monoclonal antibody. Bol Cent Panam Fiebre Aftosa. 2001; 64-67: 18-25
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