Microtitier neutralization test for the study of Foot-and-Mouth disease antibodies: brief report

Abstract

A microneutralization test (MNT) for foot-and-mouth disease (FMD) virus was described by Wagner and McVicar (4). The authors used PK15, IB-RS-2 and BHK-21 cione 13 cell lines as well as primary cultures of bovine thyroid and kidney cells. In their system, virus-serum mixtures were incubated in tubes, cells were added to these mixtures and the suspension distributed over the wells of disposable plates. The plates were incubated at 37ºC in a humidified atmosphere with 5% CO2 and then read microscopically for cytopathic effects after 48 hours of incubation. BHK-21 cells were found to be most suitable for this system.