Microtitier neutralization test for the study of Foot-and-Mouth disease antibodies: brief report
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A microneutralization test (MNT) for foot-and-mouth disease (FMD) virus was described by Wagner and McVicar (4). The authors used PK15, IB-RS-2 and BHK-21 cione 13 cell lines as well as primary cultures of bovine thyroid and kidney cells. In their system, virus-serum mixtures were incubated in tubes, cells were added to these mixtures and the suspension distributed over the wells of disposable plates. The plates were incubated at 37ºC in a humidified atmosphere with 5% CO2 and then read microscopically for cytopathic effects after 48 hours of incubation. BHK-21 cells were found to be most suitable for this system.
Prueba de microneutralización para estudios de anticuerpos de la fiebre aftosa: comunicación breve
Ferreira MEV. Microtitier neutralization test for the study of foot-and-mouth disease antibodies: brief report. Bol Cent Panam Fiebre Aftosa. 1976; 21-22: 21-24.
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