Foot-and-Mouth disease virus enzyme-linked immunoelectrotransfer blot for detection of antibodies against replicating virus in sera from persistently infected animals

Abstract

[Introduction] An enzyme-linked inmunoelectrotransfer blot (EITB) assay, was developed at the Pan American Foot-and-Mouth Disease Center, PAHO/WHO, for the detection of antibodies against replicating foot-and-mouth disease virus (FMDV) in sera from cattle with suspected persistent infection. Using only a minute quantity of sera, the test measures the levels of antibodies against some of the proteins produced by the virus when it replicates in the host.