Evaluation of three commercially-available chikungunya virus immunoglobulin G immunoassays
Date
2017-06Metadata
Show full item recordAbstract
The emergence of chikungunya virus in the Americas means the affected population is at risk
of developing severe, chronic, rheumatologic disease, even months after acute infection.
Accurate diagnostic methods for past infections are essential for differential diagnosis and
consequence management. This study evaluated three commercially-available chikungunya
Immunoglobulin G immunoassays by comparing them to an in-house Enzyme-Linked
ImmunoSorbent Assay conducted by the Centers for Disease Control and Prevention (Atlanta,
Georgia, United States). Results showed sensitivity and specificity values ranging from 92.8%
– 100% and 81.8% – 90.9%, respectively, with a significant number of false-positives ranging
from 12.5% – 22%. These findings demonstrate the importance of evaluating commercial kits,
especially regarding emerging infectious diseases whose medium
and long-term impact on the
population
is unclear. Como consecuencia de la aparición del virus del chikungunya en las Américas, la
población afectada corre el riesgo de padecer reumatismos crónicos graves, aun meses
después de la infección aguda. Es fundamental contar con métodos precisos para
diagnosticar los antecedentes de la infección a fin de elaborar un diagnóstico diferencial
y abordar las manifestaciones de la fase crónica. Se han estudiado tres inmunoensayos
comercializados de detección de inmunoglobulinas G para el diagnóstico del
chikungunya, comparándolos con el enzimoinmunoanálisis de adsorción (ELISA)
propio. Los resultados señalan valores de sensibilidad del 92,8% al 100% y de especificidad
del 81,8% al 90,9%, así como un número significativo de falsos positivos, de
entre el 12,5% y el 22%.
Translated title
Evaluación de tres inmunoensayos comercializados de inmunoglobulina G para el diagnóstico del virus del chikungunya
Subject
Category of PAHO Strategic Plan 2014-2019
Citation
De Salazar PM, Valadere A, Goodman CH, Johnson BW. Evaluation of three commercially-available chikungunya virus immunoglobulin G immunoassays. Rev Panam Salud Publica. 2017; 41:e62.
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