La prueba dot-Elisa para la detección de campo de la leishmaniasis visceral en Honduras
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The dot enzime-linked immunosorbent assay (Dot-Elisa), a rapid visually read microtechnique for serodiagnosis of human visceral lesihsmaniasis, was field-tested in a known endemic area in the Republic of Honduras. Of 305 individuals screened at a serum dilution of 1:32 positive reactions were observed in eight of nine parasitologically diagnosed visceral leishmaniasis patients who had received treatment, 13 of 45 family members of patients (a high-risk group), and eight of 244 randomly selected children in the endemic area. Cross-reactions were observed in one of three children with parasitologically confirmed cutaneous leishmaniasis and three of four adults serologically positive for Chagas'disease. End-point titrations performed on the visceral leishmaniasis sera gave reciprocal titers ranging from 512 to 8 192 which are lower than those usually encountered in untreated active cases. This test does not require electricity, and all materials are easily transportable on foot. It is rapid, simple to perform, and inexpensive, yet sensitive and relatively specific under field condidtions. It could prove to be a valuable tool for primary health care facilities and for epidemiologic survey in the many endemic areas where no serologic testing capability currently exists
Field use of the dot-Elisa test for visceral leishmaniasis in Honduras
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Pan American Health Organization; Communicable Diseases and Environmental Determinants of Health (CDE) (Washington, D.C., PAHO, 2020-12-23)In the Region of the Americas, the leishmaniases are a group of diseases caused by various species of Leishmania, which cause a set of clinical syndromes in infected humans that can involve the skin, mucosa, and visceral ...
Plan of Action to Strengthen the Surveillance and Control of Leishmaniasis in the Americas 2017-2022 Pan American Health Organization (Washington, D.C, PAHO, 2017-07)[Introduction]. The Leishmaniasis Plan of Action for the Americas is an instrument that consolidates the main lines of action to strengthen surveillance and control of the disease in the Region. Furthermore, it presents ...