Solucoes de sorbitol-gelatina e de acido glutamico-lactose na estabilizacao de preparacoes referencia do virus do sarampo
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The study described in this article was carried out for the purpose of evaluating the protective effects of two stabilizing solutions- sorbitol-gelatin and glutamic acid-lactose- on freeze-dried measles virus (Schwartz strain) with a view to the production of reference preparations in working lots. The effect of storage at -20 C and -70 C on the potency of stabilized virus suspensions, whether or not freeze-dried, was evaluated over a period of 21 months; the samples were concurrently titered for potency with a standard virus obtained from a laboratory authorized by the World Health Organization. After comparing and commenting on the different phases of the study, the types of cells and stabilizing media used, how the suspensions were stored, the titering of the stabilized viruses, etc., the authors conclude that they have demostrated a more satisfactory stabilization (r= -0.01) of freeze-dried virus suspensions stored at -20 C by sorbitol-gelatin, which is therefore regarded as an effective stabilizer for the preparation of freeze-dried reference measles virus in working lots
Sorbitol-gelatin and glutamic acid-lactose solutions as stabilizers of reference measles virus preparations
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