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Medición de los anticuerpos contra el virus de la inmunodeficiencia humana: estudio internacional en colaboración para evaluar los sueros de referencia de la OMS

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Date
s.d.
1988
Document Number
BS/88.1602
Author
Garrett, A. J
Seagroatt, V
Supran, E. M
Habermehl, K. O
Hampl, H
Schild, G. C
Metadata
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Abstract
Two preparations of human sera, one reactive against human immunodeficiency virus (HIV) and the other unreactive, were evaluated as potential international reference reagents (IRR) in an international collaborative study. Twenty-one laboratories participated and tested these and five other human sera which were found to range from highly reactive to unreactive. The proposed "positive" IRR was found to react strongly in all immunoassays and gave all the expected bands in immunoblot systems using HTLV-III, LAV-I or similar virus strains as antigens. The "unreactive" serum was judged to be negative by ELISA and immunoblots. The end-points determined by ELISAs varied considerably between laboratories, even between those using the same commercial kit. This variation was reduced somewhat when the reactivities of the samples were expressed relative to the proposed IRR
Series
Boletín de la Oficina Sanitaria Panamericana (OSP);105(5-6),nov.-dic. 1988
Subject
HIV; Anticorpos Antivirais; Ensaio de Imunoadsorção Enzimática; Cooperação Internacional; Valores de Referência; Imunoensaio; Organização Mundial da Saúde
URI
https://iris.paho.org/handle/10665.2/17658
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  • Pan American Journal of Public Health

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