La microtécnica de hemaglutinación para el diagnóstico retrospectivo de la peste humana en Zonas Rurales del Brasil
Date
1967Metadata
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The reactivation of foci of human bubonic plague in the endemic areas of Brazil during the period 1964-1966 led to checking the usefulness of the hemagglutination microtechnique test in diagnosing the disease in rural areas of the States of Bahia and Minas Gerais. Both the clinical and the bacteriological diagnoses were made in 1965 and 1966, and the blood samples used for the test came from patients who had recovered from the disease Three differente groups were tested. The first consisted of 11 persons, among whom those who had a serum titer of 1:24 or over were considered positive; the second and third groups consisted of 30 and 8 persons, respectively, among whom agglutinations observed at 1:4 and 1:8 dilutions were considered suspect, and agglutinations observed at dilutions of 1:16 or over were recorded as positive. The serological tests in all three grups were made with tanned red blood cells of sheep (Group A), formolized and tanned (Groups B and C), and sensitized with fraction 1 (F1) or Pasteurella pestis. To dilute the sera in all cases, a saline solution plus normal 5 per cent rabbit serum, inactive and adsorbed in red blood cell of sheep, were used Survey results indicated that retrospective diagnoses of human bubonic plague could be obtained through the hemagglutination test, using the microtechnique method. The test made it possible to confirm the clinical and ...(AU)
Translated title
Hemagglutination microtechnique for a retrospective diagnosis of human plague in Rural Area in Brazil
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