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dc.contributor.authorFajardo, Esther Mes_ES
dc.contributor.authorFernández, José Les_ES
dc.contributor.authorSolís, Rosa Les_ES
dc.contributor.authorPortuondo, Beatrizes_ES
dc.contributor.authorHeredia, Lorenzoes_ES
dc.contributor.authorNoroña, Martaes_ES
dc.contributor.authorUrquiza, Héctores_ES
dc.contributor.authorAmat, Martaes_ES
dc.date.accessioned2016-03-05T18:57:51Z
dc.date.available2016-03-05T18:57:51Z
dc.date.issued1996es_ES
dc.identifier.urihttps://iris.paho.org/handle/10665.2/27782
dc.description.abstractThis article describes a combination of methods- a solid-phase enzyme-linked immunosorbent assay (ELISA) combined with an ultramicroanalytical system (UMAS)- that can be used to measure tetanus antitoxin activity in human sera or plasma. The test, which is rapid and permits analysis of 78 samples of serum per reaction plate with a volume of 10 uL of diluted serum per sample, is proposed as an alternative to the traditional biologic assay in mice based on seroneutralization of a known dose of tetanus toxin. The study reported here compared these two procedures, using them both to evaluate 100 sera from de Clinical Laboratory of the General Calixto García Hospital in Havana, Cuba. The two sets of results showed a high degree of correlation (r = 0.99) when subjected to linear regression analysis (95 percent CI = 0.985-0.993). These and other findings indicate that the cheap and rapid ultramicroELISA method can perform certain tasks for which the slower and costlier traditional assay is not well suited, such as field evaluation of tetanus toxoid vaccines and identification of hyperimmune plasmas appropriate for use in producing specific antitetanus immunoglobulin (AU)en_US
dc.description.abstractThis article was also published in Spanish in the Bol. Oficina Sanit. Panam. Vol. 119(2):113-20, 1995en_US
dc.format.extentiluses_ES
dc.relation.ispartofseriesBulletin of the Pan American Health Organization (PAHO);30(1),mar. 1996en_US
dc.subjectTetanus Antitoxines_ES
dc.subjectEnzyme-Linked Immunosorbent Assayes_ES
dc.subjectImmunoenzyme Techniqueses_ES
dc.subjectBiological Assayes_ES
dc.subjectCubaes_ES
dc.titleUltramicroELISA for measuring tetanus antitoxin in human seraes_ES
dc.typeJournal articlesen_US
dc.rights.holderPan American Health Organizationen_US


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