UltramicroELISA for measuring tetanus antitoxin in human sera
MetadataShow full item record
This article describes a combination of methods- a solid-phase enzyme-linked immunosorbent assay (ELISA) combined with an ultramicroanalytical system (UMAS)- that can be used to measure tetanus antitoxin activity in human sera or plasma. The test, which is rapid and permits analysis of 78 samples of serum per reaction plate with a volume of 10 uL of diluted serum per sample, is proposed as an alternative to the traditional biologic assay in mice based on seroneutralization of a known dose of tetanus toxin. The study reported here compared these two procedures, using them both to evaluate 100 sera from de Clinical Laboratory of the General Calixto García Hospital in Havana, Cuba. The two sets of results showed a high degree of correlation (r = 0.99) when subjected to linear regression analysis (95 percent CI = 0.985-0.993). These and other findings indicate that the cheap and rapid ultramicroELISA method can perform certain tasks for which the slower and costlier traditional assay is not well suited, such as field evaluation of tetanus toxoid vaccines and identification of hyperimmune plasmas appropriate for use in producing specific antitetanus immunoglobulin (AU)This article was also published in Spanish in the Bol. Oficina Sanit. Panam. Vol. 119(2):113-20, 1995
Showing items related by title, author, creator and subject.
Metanálisis sobre la utilidad de ELISA, PCR e inmunocromatografía en el diagnóstico de chikungunya Flórez-Álvarez, Lizdany; Cardona-Arias, Jaiberth Antonio (2017-12)[RESUMEN]. Objetivo. Evaluar la utilidad de ELISA, PCR e inmunocromatografía para el diagnóstico de chikungunya. Métodos. Se realizó un metanálisis de estudios que reportaran datos de validez diagnóstica, a partir de un ...
Saborío Galo, Saira; González, Karla; Téllez, Yolanda; García, Nadezna; Pérez, Leonel; Gresh, Lionel; Harris, Eva; Balmaseda, Ángel (2017-06)Objective. To develop and evaluate serological methods for chikungunya diagnosis and research in Nicaragua. Methods. Two IgM ELISA capture systems (MAC-ELISA) for diagnosis of acute chikungunya virus (CHIKV) infections, ...
Peters, Abena; Vokaty, Alexandra; Portch, Richard; Gebre, Yitades (2017-12)[ABSTRACT]. Objective. To describe leptospirosis epidemiology, seroprevalence, and serovars among humans and animals in the Caribbean from 1979 – 2013. Methods. A retrospective study of the literature was performed on the ...